See our Privacy Policy and User Agreement for details. A small drop of blood is placed on the midline at the end of a glass slide. and It is the most common smear preparation in the hematology laboratory and Wright stain, a Romanowsky stain, is the most common dye. This test provides information about the number and shape of these cells. You can change your ad preferences anytime. BLOOD SMEAR PROCEDURE • Mix blood properly • Place a small drop of blood (about 2-3 mm) about 1 cm from the frosted end of a clean slide (slide must be completely clean) • Using a spreader (another slide), draw backwards into the drop of blood (while applying pressure) – maintain an angle of 30-45 degrees The latter will prove useful if a problem oc-curs during the staining process and/or if you wish later to send the smears to a reference laboratory. Place a small drop of blood, or one side about 1-2 cm from one end. The are two additional types of blood smear used for specific purposes. Keep the same angle all the way to the end of the smear. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. SMEAR PREPARATION 1. Preparing the smear requires attention to a number of details that help prevent contamination of the culture and ensure safety to the preparer. Precautions: Ensure that the whole drop of blood is picked up and spread. Staining the Thick blood smear with Giemsa stain ⇒ Prepare a thick blood smear on a clean and dry microscopic glass slide and air dry it. Peripheral Smear Preparation. If you continue browsing the site, you agree to the use of cookies on this website. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Then move the spreader forward rapidly over the slide. A thin peripheral blood film is thus … Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. Samples with heparin are unusable for preparation of the smears. If you continue browsing the site, you agree to the use of cookies on this website. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the … from the ddacie and todd this ppt is there, for md pathology students. Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. It highlights the procedure to be followed and the equipment required. Now customize the name of a clipboard to store your clips. Go to my Channel Page, click on "Videos". A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. However, the blood shouldn't clot when it's just moving through the body. The extra time and care taken during the field season will be rewarded later when the smears must be scanned, and parasites identified and counted. If you continue browsing the site, you agree to the use of cookies on this website. Clean slides with 70 to 90% alcohol and allow to dry. Prof. - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D. 1. 4/10/2012 Approved Lisa Senzel Asst. A poor slide is a torment. Once the needle is removed from the lesion, it is attached to an air-filled syringe and the material within the needle is gently expelled onto clean glass slide/s for smear preparation as described below. 4. Preparation involves placing a drop of well -mixed, ethylenediaminetetraacetic acid (EDTA) anticoagulated blood at one end of the slide. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. (slide preparation and reporting) 3. The most common technique of blood smear preparation is called the “wedge or push” technique. SMEAR PREPARATION 1. red blood cells, White blood cells & platelets, Iron deficiency anemia pathogenesis and lab diagnosis, No public clipboards found for this slide. Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. ⇒ After 2 minutes, add twice the amount of distilled water or Phosphate buffer solution and mix the content by swirling or by blowing gently. Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. Now customize the name of a clipboard to store your clips. What is the best time for collection of blood to demonstrate malarial parasite. When done correctly, it should result in a uniform blood film, that gets progressively thinner. Peripheral blood smear examination We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Giemsa stain - Recommended for detection and identification of blood parasites. If you continue browsing the site, you agree to the use of cookies on this website. Preparation of Blood Smear: Collection of Sample: 1. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. View A385992265_12053_13_2018_Smear Preparation and staining.ppt from PHARMACY BTY304 at Lovely Professional University. Move the spreader backward so that it makes contact with drop of blood. Examination of the Peripheral Blood Smear.docPage 1 of 7 Printed 1/14/13 Created Lisa Senzel Asst. Dr. Bahoran Singh Wear gloves. Selection of a spreader: Take one slide a spreader which has smooth edge. The blood smear test or peripheral smear test is a type of blood test that is done to procure detailed information about the number and shape of blood cells.This test focuses on the red blood cells, white blood cells and platelets. All stages of P.vivax, P.ovale, and P.malariae can be seen in smear. This ppt contain usefull information regarding different staining methods and stains with photographs...enjoy and learn it..give comments.. Looks like you’ve clipped this slide to already. Blood smear preparation and staining Prepared by: Ibtisam H. Al Aswad Amany S. Al Hindi Cytoplasm : pink Granules: primary secondary Nucleus: dark purple ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3ee768-MzBjN Making and Staining a Blood Smear A well-made blood smear is a beauty to behold, and likely to yield interesting and significant information for a research project. with different 2. Prof. - Clinical Path 1/10/2013 Replaces 5/08 HEM 3.2.5 Procedure: Examination of the Peripheral Blood Smear (How to Perform) The drop should be in the center line of the glass slide. Blood smear is prepared, stained with Leishman’s stain and cells are identified under oil immersion lens. 4. See our User Agreement and Privacy Policy. With your right hand, place the smooth clean edge of a second (spreader) slide on the specimen slide, just in front of the blood drop. Blood should be collected at or just after the height of febrile paroxysms; What stages of malarial parasites can be seen in peripheral blood smear. 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