Uniformity of sampling 4. 1. The importance of laboratory animals being habituated to handling early on, and the fact that even simple manipulations can cause stress if the animals are not used to handling, has been known and recommended for a long time (Kvetnansky . Basic handling, restraint, and other techniques will be demonstrated. Handling • Place one hand across the back of the animal with thumb behind the shoulder and the other fingers well forward on the opposite side • Lift the animal gently and support its weight with other hand placed palm uppermost under the hind quarters. In the U.S, according to Stevens (1990), there were a number of states that passed anti-cruelty laws between 1828 and 1898. See our User Agreement and Privacy Policy. The AWA became the first Federal law protecting the welfare of laboratory animals and brought the issue of stolen pets to the forefront of animal welfare concerns. 1995). Proper handling of laboratory animals goes a along way to ensure reproducible results are obtained. The adoption of these techniques will help to minimise stress for the animals and help reduce the risk of bite injuries to the handler. With the worldwide increase in biotechnology, more and more researchers are required to use animals … M pharm part 1 Pharmacology Contents: Contents Introduction Types of experimental animals used Animal handling Routes of drug administration in experimental animals Blood collection from experimental animals … India is one of the pioneering countries to institute Prevention of Cruelty to Animals Act in 1960 whereas such Act was instituted in France in 1963 and in USA in 1966. Search for more papers by this author. Restraint and Handling of Wild and Domestic Animals is an unparalleled source of information on this core skill for veterinarians, veterinary students, and technicians alike. 89–544) was signed into law by President Lyndon B. Johnson on August 24, 1966. used by all persons responsible for laboratory animals and particularly by licensees and others now working under the authority of the Animals (Scientific Procedures) Act, 1986. Laboratory Animal Handling . Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. 5. 1. Lori Gladney. Executive Guideline No. Training Programme on Laboratory Animal Management and Handling for Research Scholars Directorate of Centre for Animal Health Studies, TANUVAS, Chennai 600 051. Title: Laboratory Animal Handling Technique - Mouse Author: boshan Last modified by: alberto Created Date: 8/20/1999 7:33:05 AM Document presentation format – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 6d3aa3-Y2YyZ Handling and Restraint Rats are typically docile animals, particularly if they are routinely handled using appropriate techniques. Power and Lighting:- Lighting system be installed that provides adequate illumination while people are working in the animal rooms and a lowered intensity of light for the animals. We recommend that all … International Standards for the Care and Use of Laboratory Animals . John C. Donovan. Handling of experimental Biosafety guidelines for handling SARS-CoV specimens, by specimen type, are provided below. M. Pharm. Mr. Subhash Lal Karn First published: 01 February 2007. The use of appropriate and skilled handling is essential to ensure that animals readily accept or actively seek human contact and procedures are carried out efficiently. Knowledge, Well Presented Sign up for a LinkedIn account to learn or share your insights about any topic on SlideShare. If routine handling procedures are aversive, animals are likely to develop anxiety Laboratory confirmation of infectious diseases. Explore Dr.A.Sanyal clipboard My Clips on SlideShare, or create your own and start clipping your favorite slides. Rabbit: The body of the rabbit is closely covered with smooth hair or fur, […] of Microbiology View and Download PowerPoint Presentations on Laboratory Animal Handling PPT. See our Privacy Policy and User Agreement for details. OBJECTIVES To provide basic concept of animal handling to new user. When working in the field, personnel should work in terms of at least two people especially when involved in the physical or … TECHNIQUES. The Animal Welfare Act (Laboratory Animal Welfare Act of 1966, Pub.L. through different persons before it reaches the laboratory. No Storage: Do not store food, beverages, or medicines in refrigerators, freezers or coldrooms where radioactive materials are used or stored. M pharm part 1 Pharmacology. 14 states exempted animal … Appropriate handling and restraint techniques should be used, and training in their application should be provided to avoid injury to both animals and humans. To obtain the maximum performance from your laboratory glassware correct handling is essential. Class participants have the opportunity to practice their newly-acquired skills under the supervision of experienced trainers. MEENAKSHI M.Sc. The sessions should provide opportunities for each individual to participate in skill-building activities such as methods of handling animals and performing necessary procedures. Good Laboratory Practices. Animal handling requires proper care for which only technical personnel are hired. Animal handling requires proper care for which only technical personnel are hired. Handling Place one hand across the back of the animal with thumb behind the shoulder and the other fingers well forward on the opposite side Lift the animal gently and support its weight with other hand placed palm uppermost under the hind quarters. Öbrink ; Laboratory animal science is a science auxiliary to biological and medical research, but still a well demarcated discipline in research and teaching. Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. laboratory animals 1. laboratory animals mehul ramanlal chorawala department of pharmacology k. b. institute of pharmaceutical education and research a constituent college of kadi sarva vishwavidyalaya gh-6, sector-23 gandhinagar-382023, gujarat Posted on 6/24/2019. John C. Donovan. Handling is generally restricted to individual or group transfer from cage to cage during cage change or to transfer of animals from and to the experimental envi-ronment. 1 Animal Experiments in Medicine Animal houses, breeding of experimental animals József Kaszaki Ph.D Institute of Surgical Research HURO/0901/069/2.3.1. Animal welfare is attracting increasing interest worldwide, but particularly from those in developed countries, who now have the knowledge and resources to be able to offer the best management systems for their farm animals, as well as potentially being able to offer plentiful resources for companion, zoo and laboratory animals. Join SlideShare to discover, share, and present presentations and infographics on the world’s largest professional content sharing community. animal We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Why has this been so difficult to achieve? The laboratory staff can provide guidance when there are questions regarding sample collection and handling, as well as offering assistance in interpretation of test results. Lab safety rules and symbols Summary Several sections of the guidelines have yet to be finalised, but the Councils are now issuing those relating to housing and care of laboratory animals. Biology of the most frequently used mammals 1.1. If you continue browsing the site, you agree to the use of cookies on this website. et al., 2008). Common Laboratory Animals and Handling Techniques Anuja.M.K. NEW AND OLD EQUIPMENTS AND ACCESSORIES USED IN EXPERIMENTAL PHARMACOLOGY, Customer Code: Creating a Company Customers Love, Be A Great Product Leader (Amplify, Oct 2019), Trillion Dollar Coach Book (Bill Campbell). with experience in animal handling and animal research, or v. MD/ MS with research experience in laboratory animal handling. Care and Handling of Laboratory Animals. No public clipboards found for this slide. 12 of productive restring. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. ADVERTISEMENTS: The following points highlight the top four common laboratory animals employed in the laboratory for research purpose and for the diagnosis of the diseases. et al., 1978; Maurer . Laboratory Animal Care (OLAC) before animals are inoculated with cells that may harbor transmissible pathogens (human or murine). Temperature and humidity control:- Air conditioning is an effective means of regulating these environmental parameters for laboratory animals. Laboratory procedures Common laboratory species suffer marked stress, fear and possibly distress (indicated by the distortion of a broad range of physiological parameters) when subjected to: Handling Blood sampling Gavaging Animals do not readily habituate to these procedures over time. U.C.M.S. Personal safety begins with refraining from the following activities in laboratories, animal rooms, procedure rooms, or … To initially restrain a rat, the handler should gently grasp it around the shoulders. All laboratories using vertebrate lab animals in the United States are required by law to have a licensed veterinarian on staff and to adhere to the NIH Guide for the Use and Care of Laboratory Animals, which further stipulates that all protocols, including the sources for obtaining the animals, must be reviewed by an independent committee. This class provides an introduction to facilities, as well as an overview of the laboratory rodent in both a lecture and hands-on format. Contents: Contents Introduction Types of experimental animals used Animal handling Routes of drug administration in experimental animals Blood collection from experimental animals … General Precautions. Potential hazards include chemicals, such as commonly used sterilant … Find PowerPoint Presentations and Slides using the power of XPowerPoint.com, find free presentations research about Laboratory Animal Handling PPT 18.12.5 Animal Handling. The Laboratory Animal Medicine (LAM) unit was commissioned in 1973 with the objective of breeding, maintenance and supply of quality research animals. Animal cell culture A. The most frequently used animal species for PAb induction in the laboratory setting are the rabbit, mouse, rat, hamster, guinea pig, goat, sheep, and chicken ( Hanly et al. Extrapulmonary Specimens • The laboratory should expect to receive a variety of extrapulmonary specimens which may be ... laboratory, please review the module on biosafety within this series 35. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Storing Food & Items in Your Desk: You may store your food, water bottles, beverages, medicines, coffee mugs, eating utensils, etc. Techniques for performing parenteral injections and blood collection are addressed by separate Animal Care Services (ACS) guidelines. From tail: For collection of small amount of blood (Approximate 0.1 ml ). If you continue browsing the site, you agree to the use of cookies on this website. While this is widely accepted for both the Please click here to provide your email address to complete the login/sign-up process. Laboratory Animal Science ? ANIMAL HOUSE DESIGNING Walls and Ceilings:- Walls should be free of cracks. Here are three (3) special collections of articles curated by Editor-in-Chief Beat Riederer presents cutting-edge research on the theme of Neuroscience , Cardiovascular Research and Behavioural Science , spanning papers from the journal’s archive to the … Animal Care and Use Committee Page 1 of 5 Last revised 1/6/2014 Then, introduce the feeding tube from the pharynx in to the esophagus when the mouse is in the act of swallowing. You can change your ad preferences anytime. Rat 4. 1st Year ANIMAL HANDLING TECHNIQUES 2. Protective clothing, footwear and gloves may reduce the chance of injury when handling wild animals. Looks like you’ve clipped this slide to already. Patricia Brown. The most common site for administer antibiotic, tranquilizer and anesthetic, A common location for small amount of blood collection. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. In this assessment, OTA analyzes the scientific, regulatory, economic, legal, and ethical As with other species, hectic and jerky move-ments should be avoided. ANIMAL HANDLING Handling of Laboratory Animals – An Introduction. Clipping is a handy way to collect important slides you want to go back to later. Murray Fowler, DVM, is Professor Emeritus of Zoological Medicine at the School of Veterinary Medicine, University of California at Davis. Patricia Brown. 18.12.5 Animal Handling. A workshop/laboratory is an opportunity to gain hands-on experience. Typically, allergies to animals result from repeated exposure to an animal’s dander, urine, saliva, serum, or other body tissues. 2. Join LinkedIn. Izabella Osakwe. Laboratory animal research remains a very important part of basic research and drug development. This com… Transitional rooms at the rear ofthe laboratory are needed for receiving laboratory and animal Próxima SlideShare. Here are three (3) special collections of articles curated by Editor-in-Chief Beat Riederer presents cutting-edge research on the theme of Neuroscience , Cardiovascular Research and Behavioural Science , spanning papers from the journal’s archive to the … Mous. 18.1): Handling: Animals should be handled with care. 1st Year Laboratory animal handlers have a strict obligation to consider the safe handling and disposal of their animal waste streams. Amendments to the AWA enacted in 1970, 1976, 1985, 1990, and 2002 refined standards of care and extended coverage to animals in commerce, exhibition, teaching, testing, and research. Typically, this mayinclude a small through-the-wall ethylene oxide gas cham- ber for thecold sterilization ofheat-sensitive ma- terials (8) and a UV apparatus for decon- taminating sheets ofpaper passed out ofthe in- fectious area (13), as illustrated in Fig. Biotechnology/ Biomedical Engineering) with experience in animal handling and animal research. Rahul Maheshwari, ... Rakesh K. Tekade, in Dosage Form Design Parameters, 2018. Laboratory animals – breaded and handled in laboratory •Mice •Rat •Guinea pig •Rabbits RAT species Rattus norvegicus Wister starin is commonly used Other strains – Experimental pharmacology ... Handling of laboratory animal Subhash Karna. Rabbit 2. Laboratory Animal # 1. Aseptic Techniques: Getting Started Animal Handling Techniques 1. If you continue browsing the site, you agree to the use of cookies on this website. or iv. The Welfare of Laboratory Animals PDF.. APIdays Paris 2019 - Innovation @ scale, APIs as Digital Factories' New Machi... No public clipboards found for this slide. Most diagnostic laboratories publish user guidelines with preferred protocols for sample collection and submission, but the following broad recommendations are fairly standard. 1. blood collection techniques from laboratory animals Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Dealers in the United States. Improve quality of specimens 3. Data’s of Laboratory Animals: Table 18.1 shows important data’s on some of the animals. Laboratory Animals and Animal Tissue Disposal The use of laboratory animals in research and instruction is regulated by federal law and UW policy. Guidelines on implementing a medical surveillance system for laboratory workers are provided in Appendix F6. the increasing frequency of experiments without the use of animals, or the use of animals with particular characteristics (e.g. In 1985, Congress enacted three laws that dealt with labora-tory animals, including amendments to the Animal Welfare Act. Handling and Restraint of Small Laboratory Animals These guidelines have been developed to introduce investigative staff to procedures recommended for handling and restraint of small laboratory animals. Specimen Collection, Handling, Transport and Processing 15 . Guinea Pig 3. Purpose - This Executive Guideline is intended to provide general direction for the development and maintenance of an Occupational Health and Safety Program (OHSP) tailored to the needs of persons whose employment involves contact with laboratory animals and/or animal tissue. This is the cell culture obtained straight from the cells of a host tissue. MEENAKSHI Search for more papers by this author. Intact animal study (without sacrificing animal) Isolated organ study (by sacrificing animal) Thus every screening (OR) evaluation of pharmacological properties of drugs depends on an animal study. Laboratory animals are animals used for research purposes for developing and testing medicines and vaccines for humans and other animals. This guideline aims to improve the quality of laboratory services by improving the quality of the samples received at the laboratory. M.Sc. Now customize the name of a clipboard to store your clips. The goal of these is to provide humane treatment to animas, protect researchers, animal handlers and the environment. See our Privacy Policy and User Agreement for details. Chapter 6 - Training in Care, Handling, and Use of Laboratory Animals The IACUC is charged with warranting that the university “ensure that all scientists, research technicians, animal technicians, and other personnel involved in animal care, treatment, and use are qualified to perform their duties. This tutorial will outline recommended techniques for handling rodents, rabbits and ferrets. To comply with the Animal Welfare Ordinance and avoid mishandling of animals in research. Electric clipper, Vacuum cleaner, Scalpel, blades and handle, Tissue ,forceps x2, Scissors, Bulldog clamp, Homeostatic forceps, 3 - 000 silk suture, 16G Intravenous catheter, 50 ml Centrifuge tubes. Search for more papers by this author. Bites from rats are uncommon and will typically only occur if the animal is stressed or in pain. used by all persons responsible for laboratory animals and particularly by licensees and others now working under the authority of the Animals (Scientific Procedures) Act, 1986. Endia Ford. It is their responsibility to evaluate the hazards, assess the risks, and choose an appropriate strategy. K-J. Common Laboratory Animals and Handling Techniques Anuja.M.K. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. In this video, we will first review the basic techniques for handling rodents, and then we'll discuss the rodent restraint procedures including manual techniques and methods involving usage of … You can change your ad preferences anytime. 15. Such specimens should be labeled accordingly, and the laboratory should be alerted to ensure proper specimen handling. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. It is the main federal law in the United States that regulates the treatment of animals in research and exhibition. Hence, handling of laboratory animals has great importance in experimental pharmacology. One reason is that humans actively and religiously inhale cigarette smoke to satisfy their extraordinary craving for nicotine, while animals are affronted by this toxic mixture and will do what they can to avoid it. Now customize the name of a clipboard to store your clips. Clipping is a handy way to collect important slides you want to go back to later. Insofar as possible, labs should be species- or technique-specific, and groups should be kept small. The minimum qualification for Nominee should be: i. At the cost of their life, discoveries in science being made globally. for handling animals dosed with these hazardous materials. Rabbit (Fig. The rabbit is picked up from cage with the ears by one hand in a firm grip and another hand is placed under the hind-quarters to … Grasp the loose skin on the back of the mouse and restrain it’s tail with your ring finger and little finger. In this regard, the routine use of specialized cage squeeze mechanism is advocated for handling the larger non-human primates. use of laboratory animals. LEARNING OUTCOMES. The detailed rules for experimentation on animals were first enacted by the Ministry of Agriculture in 1968 and were implemented by a Committee set up in pursuance of Section 15(1) of the PCA Act,1960. The animals are: 1. 21, 11-11-83; Revised 7-6-16. inbred, SPF, see below), a nd the rising prices.1 1. If you continue browsing the site, you agree to the use of cookies on this website. The qualification for Nominee(s) of CPCSEA is as under: I. Primary cell culture. All over the world, animal models are used in pre-clinical research to learn more about how the body works, and to identify causes and develop treatments and cures for disorders and diseases. ., 2004). 6. 75% alcohol cotton ball for surface disinfection. The following information is a guide on the safe handling of glassware and tips on how you can optimise its performance and life span. Animal ethics is a branch of ethics which examines human-animal relationships, the moral consideration of animals and how nonhuman animals ought to be treated. Looks like you’ve clipped this slide to already. Animals were taken care and handling of the animals were in accordance with the guidelines of Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA), India. PHILLIPS ANDRUNKLE the infectious area. Search for more papers by this author. Mouse Seventy per cent of all animals used in … We were unable to log you in/sign you up. Allergens: Laboratory animal allergies and associated asthma are among the most common conditions affecting individuals who work with laboratory animals. When handling all small mammals, a firm but gentle approach is advisable. Although there were state initiatives to protect laboratory animals, it was many years until there was a National law to protect laboratory animals in the U.S. If proper handling does not take place at these stages the quality of the sample suffers. Ultimately the lab director (faculty/lab manager/supervisor) is responsible for the safety of anyone entering their laboratory – faculty, staff, student, or visitor. this presentation accounts for handling, caring , feeding, common infections, different routes of injection and disposal of laboratory animals. Rahul Maheshwari, ... Rakesh K. Tekade, in Dosage Form Design Parameters, 2018. – 17th October 2011 Several sections of the guidelines have yet to be finalised, but the Councils are now issuing those relating to housing and care of laboratory animals. Please refer to OLAC’s policy “Testing Biologicals Used in Laboratory Rodents” for additional information. Dept. Laboratory animals are inevitably subjected to human contact throughout their lives, during both husbandry and experiments. The cells dissociated from the parental tissue are grown on a suitable container and the culture thus obtained is called primary cell culture. GLP: GOOD LABORATORY PRACTICE ... GLP is a formal regulation that was created by the FDA ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 567f5-ZDc1Z If you continue browsing the site, you agree to the use of cookies on this website. See our User Agreement and Privacy Policy. Laboratory Animals: Regulations and Recommendations for the Care and Use of Animals in Research, Second Edition, is the only publication to offer a global compilation of standards on the care, welfare and use of animals in research.The book provides updated information that will be of great interest to professionals across laboratory animal science and biomedical research. Handling of laboratory animals and ethics Introduction: Laboratory animals such as mice, rats and guinea pigs are most commonly used experimental animals for study of different biological activities in health and disease. The first study to show a robust increase in lung cancer in an animal model of cigarette smoke inhalation appears in this issue of Carcinogenesis ( 1 ), more than 50 years after the initial epidemiologic studies linking smoking and lung cancer in humans ( 2 , 3). Therefore, training for proper handling of mice and rats is required to minimize these effects on animals.